Aggregation of amphiphilic pullulan derivatives evidenced by online flow field flow fractionation/multi-angle laser light scattering

摘要

Size-exclusion chromatography (SEC) is a useful steric separation technique for the analysis of water-soluble polysaccharides in aqueous solutioni. However, in the case of amphiphilic derivatives, the usefulness is limited because of interactions between hydrophobic segments and the stationary phase. Alkyl-bearing pullulans differing from the extent and the length of alkyl groups were characterized using flow-field flow fractionation with on-line coupling multi-angle laser light scattering (F~4/MALLS). Comparison of SEC and F~4 is presented adn the interest of F~4 in the field of amphiphilic derivatives is demonstrated.