Determination of 4-nonylphenol,nonyphenol monoethoxylate,nonylphenol diethoxylate and other alkylphenols in fish and shellfish by high-performance liquid chromatography with fluorescence detection

摘要

A simple and highly sensitive method is described for the HPLC determination of 4-nonyiphenol (NP), 4-nonyiphenol ~no- (NPiEO) and diethoxylates (NP2EO) in fish and shellfish together with bisphenol A (BPA), 4-tert.-butylphenol (BP’~ 4-tert.-octylphenol (OP). The NP, NP1EO, NP2EO and other alkyiphenols in the samples are extracted with acetonitrile I the lipid in the sample extract is eliminated by partitioning between hexane and acetonitrile. After Florisil PR clean-up sample extract is analyzed by HPLC with a fluorescence detection. Recoveries in Japanese smelt, carp and corbicura are .8—84.3% for NP, 83.5—84.3% for NP1EO, 90.5—96.2% for NP2EO, 70.7—72.9% for BPA. 7 1.0—73.4% for BP and I —83.2% for OP spiked at 0.5 jig each chemical per 5 g of the fish and shellfish samples. The detection limits are 2 nglg NP, NPIEO and NP2EO, and lng/g for BPA, BP and OP.