Liquid chromatographic-tandem mass spectrometric method for the analysis of a neurokini-1 antagonist and its metabolite using automated solid-phase sample preparation and automated data handling and reporting

摘要

Phannacokinetic studies playa vital role during the development of new phannaceutical substances. Data presented iemonstrate an accurate, precise and robust assay for a neurokinin-l receptor antagonist and its metabolite with IPLC-MS-MS. Sample preparation is performed by solid-phase extraction (SPE) in the 96-well plate format. This process is lIlly automated with a Tecan Genesis TM pipetting system using its standard robotic manipulator ann (ROMA). All ilstruments are fully integrated in a study oriented laboratory information system (LIMS) with an Oracle database that ommunicates bi-directional with the analytical equipment. Finally, the results are reported by push button operation.