Assay method for the perfluorooctyl bromide (perflubron) in rat blood by gas chromatography-mass spectrometry

摘要

This paper describes a GC-MS method (SIM mode) for the analysis of perfluorooctyl bromide (perflubron,I) in rat blood.The chromatographic separation was performed by injection in the split mode using a CP-select 624 CB capillary column. Following destruction of the emulsion by addition of ethanol,the analytical procedure involves a liquid-liquid extraction with 1,1,2-trichlorotrifluoroethane. The bis(F-brtyl)ethene (II) was used as internal standard. Observed retention times were 3.22 min for I and 2.32 min for II. Two calibration curves were used; linear detection responses were obtained for concentrations ranging from 0.009 to 0.9 mg/ml and from 0.9 to 13.5 mg/ml. The extraction efficiency averaged 50% for I and 93% for II. Precision ranged from 0.7 to 14%,and accuracy was between 91 and 109%. The limit of quantification was 9 #mu#g/ml. The method validation results indicate that the performance characteristics of the method fulfilled the requirements for assay method for use in pharmacokinetic studies.