Optimized analytical method for cyclosporin A by high-performance liquid chromatography-electrospray ionization mass spectrometry

摘要

The Micromass Platform LCZ mass detector parameters were optimized for simultaneous recording of the protonated (CsA～H~+), sodium adduct (CsA-～Na~+) and potassium adduct (CsA～K~+) of cyclosporin A eluted from a Symmetry Shield RP8 column. Te optimized procedure allows a precise analysis of CsA in whole blood or serum without removal of salts prior to analysis. hte ratio of the three forms of CsA varied depending on the assay condition and the types of specimens being analyzed. The summation of three ionic forms of CsA detected by LC-ESI-MS is a reliable and simple method to assess CsA concentraiton in the blood.