Determination of esmolol in serum by capillary zone electrophoresis and its monitoring in course of heart surgery

摘要

A new Capillary Zone Electrophoresis (CZE) procedure for determination of esmolol,an ultra0short-acting #beta#-blocker,in serum was developed.Dichloromethane was applied as a deproteination agent and it was used also for the inactivation of erythrocytal esterase and in the same time for the extraction of esmolol from blood.The re-extraction of esmolol from organic phase to water phase was performed by 0.01 M HCl.An aliquot of 200 ml of acid aqueous phase was used for the injection and analysis.CZE determination was done in 50 mM phosphate buffer (pH=8.0) with detection at 222 nm.The concentration detection limit of esmolol in serum was 0.051 #mu#g/ml.This method was applied in an extensive heart surgery experiment on pigs (Sus scrofa).