Simple and reliable high-performance liquid chromatography fluorimetric procedure for the determination of amphetamine-derived designer drugs

摘要

The paper describes a HPLC-fluorimetric procedure for the determination of methylenedioxyamphetamine,methylenedioxymethamphetamine,methylenedioxyethamphetamine and N-methyl-1-(1,3-benzodioxol-5-yl)-2-butanamine in urine,serum,saliva and street samples,that features interesting advantages over other procedures previously described. The method requires a very small sample volume (100 #mu#l) and no extraction,lacks matrix effect,and is not time consuming. Linearity was in the range 50-1000 ng/ml regardless of matrix. Sensitivity and detection limit were 50 ng/ml and 10 ng/ml,respectively,but they may reach 10 ng/ml and 2 ng/ml if a slight modification is introduced in the procedure. Intra- and inter-day precision were always within 5% and 8%,respectively. Recovery was satisfactory for all matrices.The described procedure could be successfully used for clinical,epidemiological and forensic applications.