Rotine analysis of amphetamine class drugs as their naphthaquinone derivatives in human urine by high-performance liquid chromatography

摘要

We describe a simple HPLC method which is suitable for the routine confirmation of immunoassay positive amphetamine urine samples. The precolumn derivisation method employing dodium naphthaquinone-4-sulphonate was found to have adequate sensitivity,selectivity and precision for the measurement of amphetamine,methamphetamine,3,4-methyl-enedioxymethamphetamine (MDMA),3,4-methylenedioxyamphetamine (MDA),and 3,4-methylenedioxyethylamphetamine (MDEA) at 500 #mu#g/1 cutoff level for confirmatory analysis of amphetamines in urine. The specificity of the method is endanced by detecting the peaks at two different wavelengths.The ratios of the peak heights measured at the two wavelengths were different for each of the 5 amphetamines analysed.There was nointerference from other phenylethylamine analogues that are commonly found in “over the counter” preparations.The HPLC method is compared to a commercial TLC system for detecting amphetamines in urine of drug abusers attending drug rehabilitation programmes.The HPLC confirmatory method described is a viable alternative to GC or to the more complex and costly GC-MS techniques for confirming amphetamine,methamphetamine,MDMA,MDA and MDEA in urine of drug abusers especially when used in a clinical care setting