Utility of droplet digital PCR for the quantitative detection of polyomavirus JC in clinical samples

Giovannelli Irene; Ciccone Nunziata; Vaggelli Guendalina; Della Malva Nunzia; Torricelli Francesca; Rossolini Gian Maria; Giannecchini Simone

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Utility of droplet digital PCR for the quantitative detection of polyomavirus JC in clinical samples

机译：液滴数字PCR用于定量检测临床样品中多瘤病毒JC的效用

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Background: Quantitative PCR (qPCR) is the standard molecular method for detection of polyomavirus JC (JCPyV) DNA reactivation in serum and cerebrospinal fluid (CSF) in patients at risk of progressive multifocal leukoencephalopathy (PML). Recently, digital PCR has shown potential benefits over qPCR in viral diagnostics.

机译：背景：定量PCR（qPCR）是用于检测患有进行性多灶性白斑脑病（PML）的患者血清和脑脊液（CSF）中多瘤病毒JC（JCPyV）DNA活化的标准分子方法。最近，数字PCR在病毒诊断中已显示出优于qPCR的潜在优势。

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《Journal of clinical virology: The official publication of the Pan American Society for Clinical Virology》 |2016年第null期|共6页
作者
Giovannelli Irene; Ciccone Nunziata; Vaggelli Guendalina; Della Malva Nunzia; Torricelli Francesca; Rossolini Gian Maria; Giannecchini Simone;
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正文语种 eng
中图分类医学微生物学（病原细菌学、病原微生物学）;
关键词
ddPCR; qPCR; Polyomavirus JC; PML diagnostics;

机译：ddPCR;qPCR;多瘤病毒JC;PML诊断;

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1. Utility of droplet digital PCR for the quantitative detection of polyomavirus JC in clinical samples [J] . Giovannelli Irene, Ciccone Nunziata, Vaggelli Guendalina, Journal of clinical virology: The official publication of the Pan American Society for Clinical Virology . 2016,第Null期

机译：液滴数字PCR用于定量检测临床样品中多瘤病毒JC的效用
2. Droplet digital PCR (ddPCR) vs quantitative real-time PCR (qPCR) approach for detection and quantification of Merkel cell polyomavirus (MCPyV) DNA in formalin fixed paraffin embedded (FFPE) cutaneous biopsies [J] . Arvia Rosaria, Sollai Mauro, Pierucci Federica, Journal of Virological Methods . 2017,第期

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Utility of droplet digital PCR for the quantitative detection of polyomavirus JC in clinical samples

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