首页> 外文期刊>Journal of clinical virology: The official publication of the Pan American Society for Clinical Virology >Evaluation of HIV/AIDS diagnostics kits and formulation of a testing strategy for Pakistan
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Evaluation of HIV/AIDS diagnostics kits and formulation of a testing strategy for Pakistan

机译:评估艾滋病毒/艾滋病诊断试剂盒并制定巴基斯坦检测策略

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Background: Rapid diagnosis of HIV/AIDS enables the development of prevention and treatment programmes but accurate, reliable and cost effective testing strategies should be used for testing of HIV/AIDS from a large population. Objective: To evaluate the performance and effectiveness of three assays for the diagnosis of HIV in comparison with Western blot and to formulate an alternative cost-effective confirmatory approach for HIV diagnosis. Study design: 472 specimens (serum) from a Pakistani population were evaluated. Two rapid HIV testing kits (Capillus, SD Bioline) and one ELISA (Vironostika Ag/Ab) kit were used to detect HIV. Results were compared with Western blot against which sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of all HIV assays were assessed. Results: 280/472 (59.3%) of the samples were positive for antibodies against purified HIV-1 viral proteins. The sensitivity of SD Bioline and Vironostika ELISA was 100% (95% CI; 98-100) while that of anti-HIV Capillus? kit was 94.6% (95% CI; 91-96.8). The specificity of the Vironostika ELISA and anti-HIV Capillus? kit was 100% (95% CI; 97-100) while specificity of SD Bioline was 98.4% (95% CI; 95-99). PPV was 100% (95% CI; 98-100%) for the anti-HIV Capillus? and Vironostika ELISA and 98.9% (95% CI; 96-99%) for SD Bioline. NPV for SD Bioline and Vironostika ELISA was 100% (95% CI; 98-100%) and 92.7% for anti-HIV Capillus? (95% CI; 88-96%). Conclusion: The sensitivity and specificity of all three kits were satisfactory compared to Western blot and could be used for effective diagnosis of HIV/AIDS in Pakistani population. ? 2013 Elsevier B.V.
机译:背景:对HIV / AIDS的快速诊断有助于制定预防和治疗计划,但应使用准确,可靠和具有成本效益的检测策略对大量人群的HIV / AIDS进行检测。目的:与Western blot相比,评估三种检测HIV诊断方法的性能和有效性,并制定出另一种具有成本效益的HIV确诊方法。研究设计:对来自巴基斯坦的472个标本(血清)进行了评估。使用两种快速HIV检测试剂盒(Capillus,SD Bioline)和一种ELISA(Vironostika Ag / Ab)试剂盒检测HIV。将结果与蛋白质印迹法进行比较,据此评估所有HIV检测的敏感性,特异性,阳性预测值(PPV)和阴性预测值(NPV)。结果:280/472(59.3%)样品的抗纯化HIV-1病毒蛋白抗体呈阳性。 SD Bioline和Vironostika ELISA的敏感性为100%(95%CI; 98-100),而抗HIV Capillus?试剂盒为94.6%(95%CI; 91-96.8)。 Vironostika ELISA和抗HIV Capillus的特异性?试剂盒为100%(95%CI; 97-100),而SD Bioline的特异性为98.4%(95%CI; 95-99)。抗HIV Capillus的PPV为100%(95%CI; 98-100%)。以及Vironostika ELISA和SD Bioline的98.9%(95%CI; 96-99%)。 SD Bioline和Vironostika ELISA的NPV为100%(95%CI; 98-100%),而抗HIV Capillus?的NPV为92.7%。 (95%CI; 88-96%)。结论:与Western blot相比,这三种试剂盒的敏感性和特异性均令人满意,可用于对巴基斯坦人群中的HIV / AIDS进行有效诊断。 ? 2013年Elsevier B.V.

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