Interfacial Properties betweenAqueous and Organic Phases in AOT Reversed Micellar System for lysozyme Extraction

摘要

The interfacial tensions of several solutions of reversed micellar systems used in lysozyme extraction were measured by drop weight and pendant drop methods. The organic phase was 2,2,4-trimethylpentane containing sodium bis(2-ethylhexyl)sulfosuccinate (AOT), and the aqueous phase was a solution of lys- ozyme and KCI, NaCI or CaCI2. The effects of salt concentration and salt type on the interfacial tension were studied. The hydration of cation associated with AOT affects interfacial properties to some extent. At the same ionic strength, the interfacial flexibility decreased with type of salt, NaCI > CaCI2 > KCI. This order corresponds to that of the cation's hydration number. Overall mass-transfer coefficients of lysozyme were also measured under various salt concentrations of three salt systems and were expressed by a single curve when plotted against interfacial tension.