Determination of the Subunif Molecular Moss and Composition of Alcohol Dehydrogenose by SDS-PAGE

摘要

Polyacrylamide gel electrophoresis (PAGE) is a versatile biochemical technique that can be used to monitor specific proteins during purification, cloning (1), or changes in gene expression (2). It can be used to determine the subunit molecular mass (1, 3), subunit composition and stoichiometry (3, 4), and the isoelectric point (3, 5) of proteins. Gel electrophoresis can be used as a preparative technique to purify native proteins (6) or to isolate individual subunits (6, 7). In combination with immunoprecipitation and autoradiography, it has been used to investigate the synthesis, sorting, and processing of proteins (7, 8). In tandem with Western blotting, it is useful for identifying specific proteins in complex mixtures (9).