The Binding Constant for Complexation of Bilirubin to Bovine Serum Albumin

摘要

Growing emphasis on biochemistry tracks/options for undergraduate majors has bled to the need for suitable experiments for the laboratory component in biophysical chemistry (1). Instructionally, these should focus on the application of fundamental concepts of physical chemistry to systems of biological interest, preferably using commercially available and reasonably robust chemicals. It is also desirable to include instrumentation commonly employed in biochemical research. This paper summarizes the design of an experiment on the complexation of bilirubin to bovine serum albumin using molecular fluorescence measurements.