Quantitative Determination of DNA–Ligand Binding:Improved Data Analysis

摘要

Eamonn Healy recently published in this Journal (1) a fine undergraduate biochemistry laboratory project on the binding of the fluorescent ligand DAPI (4′,6-diamidino-2-phenylindole) to DNA. Students use a Scatchard analysis of fluorescence intensity to determine the maximum binding capacity and equilibrium binding constant for the DAPI–DNA adduct. For the benefit of faculty who plan to adopt this unique project, I would like to make a few suggestions to improve the data analysis.