Prevalence of Trypanosoma evansi in camels through polymerase chain reaction and haematocrit centrifugation technique in Punjab (Pakistan).

摘要

The prevalence of Trypanosoma evansi infection among camels in Punjab (Pakistan) was studied using haematocrit centrifugation technique (HCT) and polymerase chain reaction (PCR). Two different primer pairs (one specific for T. evansi and T. brucei) were used in the study. Among the 100 camels of either sex and all ages from different localities of Punjab, the prevalences were 4, 9 and 13% under haematocrit centrifugation technique, PCR primer-1 and PCR primer-2, respectively. The observations establishes evidence of the usefulness of PCR as a diagnostic tool for epidemiological studies.