Polymorphonuclear leukocytes mainly contribute to human herpesvirus-6 load in the peripheral blood of patients. Diseases Society of America

摘要

As a first approach, we evaluated HHV-6 DNA load in paired PMNL and PBMC samples obtained from 20 patients with suspected HHV-6 infection. PMNLs and PBMCs were isolated with the anti-CD15 magnetic bead purification method and Ficoll gradient separation, respectively. The HHV-6 genome was detected more frequently in PMNLs (in 18 of 20 samples) than in PBMCs (in 9 of 20 samples). Moreover, the median HHV-6 DNA load was higher in PMNLs than in PBMCs (2049 copies/1 X 10~6 cells vs. 120 copies/ 1 X 10~6 cells (figure 1A).