The NMR structure of the TC10-and Cdc42-interacting domain of CIP4

摘要

Translocation of CIP4 is mediated by association with GTP-loaded TC10 localized at the plasma membrane. Insulin-stimulated activation of TC10 is indispensable for CIP4 translocation (Chang et al. 2002). Since the TC10 and Cdc42 binding region of CIP4 does not contain a known interaction motif that includes the CRIB (Cdc42 and Rac Interactive Binding) region (Chang et al. 2002), a plausible interaction mechanism remains elusive. Here, we report the solution structure of CIP4332–425 and study the mode of interaction of CIP4332–425 with both TC10 and Cdc42.