Quantitative assessment of human erythrocyte membrane solubilization by Triton X-100.

摘要

We report here a quantitative analysis of the interaction of the non-ionic surfactant Triton X-100 with human erythrocyte membranes. By applying a classical treatment for the interpretation of the action of surface active compounds to the hemolytic curves, we could calculate parameters such as R(e)-the effective surfactant/lipid molar ratio for erythrocyte membrane saturation (R(e)(sat)) and total lysis (R(e)(sol))-and K(b), the binding constant of Triton X-100 to human erythrocyte membranes. The K(b) (5900 M(-1)) and R(e) (1.58 and 2.14) values presented here are in good agreement with literature data for Triton X-100 solubilization of model phospholipid membranes.