A method for glyoxylic acid production using cells of Alcaligenes spGOX373

摘要

A microorganism capable of producing glyoxylic acid from glycolic acid was obtained by enrichment culture with medium containing 1,2-propanediol. It was identified as a member of the genus Alcaligenes. The maximal activity of the enzyme responsible for glyoxylic acid production by this strain was obtained by culturing at pH 5-6 for 3 days. The optimum conditions of cell-reaction for accumulating high concentration of glyoxylic acid were investigated using lyophilized cells of 3 day cultivates: Approximately 0.65 M glyoxylic acid was produced from 1.0 M glycolic acid by incubating at 20 degrees C for 15 days in 1.0 M TES-NaOH buffer, pH 7.0. In addition, the production of glyoxylic acid was improved to 0.95 M by keeping the reaction pH at neutral region. The method proposed here is more advantageous for glyoxylic acid production than enzymatic methods. (C) 1999 Published by Elsevier Science B.V. All rights reserved.