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Gas holdup, foaming and oxygen transfer in a jet loop bioreactor with artificial foaming media and yeast culture

机译:带有人造泡沫介质和酵母培养物的射流回路生物反应器中的气体滞留,泡沫和氧气转移

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A concentric draft tube jet loop bioreactor (10.5 m3) was used to study the influence of aerated liquid height (above the draft tube) on the amount of surfactant addition allowable without foaming. Sodium lauryl sulfate (SLS) and defatted soybean flour in tap water were used as model artificial media. The amount of surfactant required to develop foaming and the maximum gas holdup achieved prior to foaming were notably influenced by aerated liquid height. Decreasing the aerated liquid height from 1.50 to 0.05 m increased the amount of SLS allowed without foaming from 2.2 to 12.1 g, the gas holdup in the riser from 0.18 to 0.31 and the gas holdup in the downcomer from 0.12 to 0.25. Similar behavior was observed for soybean flour. Decreasing the aerated liquid height from 1.45 to 0.05 m increased the amount of soybean flour allowed without foaming from 822 to 3200 g, the gas holdup in the riser from 0.17 to 0.26 and the gas holdup in the downcomer from 0.10 to 0.19. Data from a representative continuous yeast culture are reported to show that operation at low aerated liquid heights (0.5 m) can also be used to produce a culture broth with large gas holdup and oxygen transfer but without foaming.
机译:使用同心引流管射流环式生物反应器(10.5立方米)研究充气液体高度(引流管上方)对表面活性剂添加量的影响(不产生泡沫)。将自来水中的十二烷基硫酸钠(SLS)和脱脂大豆粉用作模型人工培养基。产生泡沫所需的表面活性剂的量和在泡沫产生之前达到的最大气体滞留量明显受充气液体高度的影响。将充气液高度从1.50降低到0.05 m,不发泡时允许的SLS量从2.2升高到12.1 g,提升管中的气体滞留量从0.18增加到0.31,降液管中的气体滞留量从0.12增加到0.25。对于大豆粉观察到类似的行为。将充气液的高度从1.45降低到0.05 m,不发泡时允许的豆粉量从822 g增加到3200 g,提升管中的气体滞留量从0.17到0.26,下降管中的气体滞留量从0.10到0.19。据报道,来自代表性的连续酵母培养物的数据表明,在低充气高度(0.5 m)下操作也可用于生产具有高气体滞留率和氧气转移但不发泡的培养液。

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