Seed-borne biocontrol agents for the management of rice sheath rot caused by Sarocladium oryzae (Sawada) W. Gams & D. Hawksw.

摘要

All the antagonist isolates (10 isolates of Pseudomonas fluorescens, 15 of Bacillus subtilis, and 3 of Trichoderma viride) obtained from rice seeds showed in vitro inhibitory effect on S. oryzae, ranging from 10.90 to 82.18%. B. subtilis isolate 9 was found to be highly effective in inhibiting the mycelial growth of S. oryzae by 82.18%. The biological control agents significantly reduced S. oryzae seed infection and increased seed germination and seedling vigour. Field trials conducted at four different locations (western, southern, northeastern and Cauvery delta) in Tamil Nadu, India, during rabi 2004 indicated that the seed-borne S. oryzae in rice could be successfully managed by seed treatment (at 10 g/kg), followed by foliar application (at 1%) at boot leaf stage with B. subtilis or P. fluorescens..