Detection and Cloning of the Gene Encoding a Protein Produced by Nonpathogenic Mutants of Fusarium oxysporum

摘要

Treatment of Fusarium oxysporum with 5-azacytidine,a potent inhibitor of DNA methylation,induced nonpathogenic mutants. Analysis of the protein expression pattern by two-dimensional gel electrophoresis revealed a protein that is present in yeast-form cells of the mutants but absent in those of the wile-trpe strain.N-terminal amino acid analysis indicated that this protein is identical to a region of a polypeptide encoded by a cDNA clone,sti35,previously identified as a heat shock gene in F.oxysporum.A genomic clone for sti35.The analysis also revealed the presence of a leader sequence composed of 27 amino acid residues,which shares a common amino acid composition with leader sequences of the proteins located in the mitochondrial matrix.Different expression patterns of sti35 in the mutants and wild-type strain were demonstrated.