Expression of a Major House Dust Mite Allergen Gene from Dermatophagoides farinae in Lotus japonicus ccession Miyakojima MG-20

摘要

Transformation of a model legume Lotus japonicus accession Miyakojima MG-20 was examined using Agrobactenum tumefaciens with a binary expression vector.Using the improved transformation method,we introduced a major allergen gene from a house dust mite,Derf 1,into MG-20.Analyses by Southern hybridization,reverse transcription (RT)-PCR,and Western blotting showed that the Der f l gene was integrated into the genome of L.japonicus,expressing the gene product in the Tl lines.Our results imply future application of oral allergen-specific im-munotherapy using legume plants.