Effects of Culture and Medium Conditions on Hydrogen Production from Starch Using Anaerobic Bacteria

摘要

The influences of initial pH (4.0-9.0),iron concentration (1.2-100 mg/l),nitrogen concentration (NH_4HCO_3,0.56-11.28 g/l) and starch concentration (2-32 g/l) on hydrogen production and volatile fatty acid (VFA) production were studied.Batch experiments using starch as a substrate and mixed bacteria were conducted at 35deg C.The optimum pH,iron concentration and nitrogen concentration for hydrogen production at a starch concentration of 15 g/l were 7.0-8.0,Fe~(2+) 10 mg/l and NH4HCO3 5.64 g/l,respectively.The typical VFAs were acetate,propionate and butyrate,with acetate as the major component.The maximum specific hydrogen production rate of 146ml/g-VSS-d (volatile suspended solids,VSS) and the hydrogen yield of 178 ml/g-starch were obtained at a starch concentration of 15 g/l under optimum environmental conditions.The maximum hydrogen yield of 194 ml/g-starch was obtained at a starch concentration of 2 g/l.The maximum specific hydrogen production rate of 237 ml/g-VSS centre dot d was found at a starch concentration of 24 g/l.