pH-Sensitive Eudragit S-100 Coated Chitosan Nanoparticles of 5-Amino Salicylic Acid for Colon Delivery

摘要

Site-specific colonic drug delivery system of 5-ASA nanoparticles were prepared and coated with pH sensitive polymer. Chitosan nanoparticles (CTNP) bearing 5-Amino salicylic acid (5-ASA) were prepared, by ionotropic gelation method, nanoparticulate dosage form consisting of a hydrophobic core enteric coated with pH-dependent polymer Eudragit S-100 by solvent evaporation method, for the effective delivery of drug to the colon for treatment of ulcerative colitis. CTNP and Eudragit chitosan nanoparticles (ECTNP) was characterized for shape and surface morphology of nanoparticles by scanning electron microscopy (SEM) appeared to be spherical in shape and the particle size, size distribution and polydispersity index of the drug loaded nanoparticles were assessed by photon correlation spectroscopy using Zeta Sizer mean size was found to be around 159 nm and 501 nm with optimum value of zeta potential was found to be 22 +/- 1.82 and -34.5 +/- 0.8 respectively. The in vitro drug release was investigated using USP dissolution test apparatus in different simulated GIT fluids showed promising release in colonic pH. These data confirmed the CTNP have been developed. The in vitro hennolysis study showed that the complex was non-hemolytic. Thus it can be concluded that the ECTNP may be a promising candidate in colon delivery.