Anti C-Reactive Protein Immobilization Onto Poly(glycidyl Methacrylate) Microspheres and its Use for Diagnosis

摘要

A method for a polymeric carrier based test was developed for the diagnosis of C-reactive protein (CRP) as an alternative to commercial kits.Poly(glycidyl methacrylate) (PGMA) particles were synthesized by dispersion polymerization method from the glycidyl methacrylate (GMA) monomer.The monomer transformation value was 88.67% by weight.In the first step the epoxide rings were opened to form amine groups on the particle surfaces.The optimized conditions were found for the antigen to be immobilized onto the surface-modified microspheres.Agglutination tests were carried out under in vitro conditions with the anti-human CRP immobilized microspheres with samples from patient serum.Relatively high sensitivity was obtained with a short reaction time between doing the test after receiving patient serum compared with the selected commercial kit now in use.By referring to the agglutination intensity scores,using only 10 mg/L samples of plasma,the CRP concentration was detectable with these new kits.