In vitro mitochondrial effects of PK 11195, a synthetic translocator protein 18 kDa (TSPO) ligand, in human osteoblast-like cells

摘要

The role of the TSPO in metabolism of human osteoblasts is unknown. We hypothesized that uman osteoblast metabolism may be modulated by the TSPO. Therefore we evaluated the presence of TSPO in human osteoblast-like cells and the effect of its synthetic ligand PK 11195 on these cells. The presence of TSPO was determined by [~3H]PK 11195 binding using Scatchard analysis: Bmax 7682 fmol/ mg, Kd 9.24 nM. PK 11195 did not affect significantly cell proliferation, cell death, cellular viability, maturation, [~(18)F]- FDG incorporation and hexokinase 2 gene expression or protein levels. PK 11195 exerted a suppressive effect on VDAC1 and caused an increase in TSPO gene expression or protein levels. In parallel there was an increase in mitochondrial mass, mitochondrial ATP content and a reduction in m collapse. Thus, it appears that PK11195 (10~(?5) M) stimulates mitochondrial activity in human osteoblast-like cells without affecting glycolytic activity and cell death.