Ontological evaluation of transcriptional differences between sperm of infertile males and fertile donors using microarray analysis

摘要

Purpose To catalogue Gene Ontology terms in the sperm of infertile human males vs. donors of proven fertility by analyzing five samples from each of the two groups (five aliquots from fresh sperm and five post-swim-up). Methods Microarray technology was employed to study the mRNA profile of both fresh and post-swim-up pooled samples from infertile males and donors.Results Genes that were differentially expressed in the two populations and expressed in only one of two were analyzed to determine the gene products in terms of their associated Gene Ontology terms. Each group presented a different number and pattern of Gene Ontology terms.