The effects of IVF aspiration on the temperature, dissolved oxygen levels, and pH of follicular fluid.

摘要

PURPOSE: To investigate the effects of IVF aspiration on the temperature, pH, and dissolved oxygen of bovine follicular fluid. METHODS: The temperature was monitored at various positions in an aspiration kit. Dissolved oxygen and pH were measured before and after aspiration. RESULTS: The temperature of follicular fluid dropped by 7.7 +/- 1.3 degrees C upon aspiration. Dissolved oxygen levels rose by 5 +/- 2 vol.%. The pH increased by 0.04 +/- 0.01. CONCLUSIONS: The temperature change was attributed mainly to evaporation of fluid in the collection tube. Changes in dissolved oxygen levels and pH were due to contact with air. Standard vacuum-based aspiration may induce changes in follicular fluid, which could be detrimental to oocyte health and affect attempts to correlate chemical characteristics with oocyte quality.