Application of RP-HPLC-diode array detector after SPE to the determination of pesticides in pepper samples.

摘要

Application of HPLC-diode array detection (DAD) after SPE for identification and quantitative analysis of pesticides in red and green pepper samples is demonstrated. An HPLC procedure on an RP column (C18) was developed for analysis of selected pesticides from different chemical groups: metamitron, metalaxyl, linuron and prometryn. Average recoveries for C18 Polar Plus cartridges and solvents by the proposed RP-HPLC-DAD method after SPE are presented. Average recoveries from the spiked samples and the SDs were 22.5 +or- 2.2, 138.0 +or- 4.1, 78.6 +or- 2.8, and 109.2 +or- 2.3% for metamitron, metalaxyl, linuron, and prometryn, respectively, at concn. of 7 mug/g in the plant material. Efficiency of the SPE procedure was evaluated using real food samples. Quantities of prometryn, linuron, metalaxyl and metamitron determined were in the ranges 0.02-2.24 (n = 24), 0.08-1.01 (n = 9), 1.61-2.28 (n = 4) and 0.05-1.07 mug/g (n = 3), respectively, in plant material sampled in 2011. The method was validated for precision, repeatability and accuracy.