Fast and High Throughput Screening of Amantadine in Chicken Muscle by Extractive Derivatization with Pentafluorobenzoyl Chloride and Gas Chromatography/Negative Ion Chemical Ionization-Tandem Mass Spectrometry

摘要

A fast and high throughput screening method for the determination of amantadine (ADA) in chicken muscle is presented. After acidic extraction and extractive derivatization with pentafluorobenzoyl chloride, ADA was analyzed directly without any preconcentration by GCegative ion chemical ionization-tandem MS/MS. With the selective MS/MS detection, ADA in chicken muscle can be detected at concentrations well below 1 mu g/kg. Quantification of ADA in chicken muscle was accomplished by using matrix-matched calibration with memantine as an internal standard. The method was validated with ADA fortified chicken muscle at 1, 2, and 5 mu g/kg. The recoveries ranged from 89 to 95%, and RSDs were <= 10% in all cases. The estimated LOD and LOQ in chicken muscle were 0.020 and 0.054 mu g/kg, respectively. This method is rapid, robust, and suitable for routine monitoring of ADA residues in chicken muscle samples