A simple technique for consistently obtaining large single crystals of hen egg-white lysozyme in a concentration gradient of NiCl2

摘要

A simple protocol is reported for consistently obtaining large single crystals of hen egg-white lysozyme (HEWL) greater than 2 mm in the longest dimension. Comparative crystallization experiments with different sources of HEWL showed that the simple addition of glass capillaries, crystal hangers, reduced excessive nucleation and resulted in large single crystals regardless of the commercial source of HEWL.