Selected fructooligosaccharide (1-kestose, nystose, and 1F-beta-fructofuranosylnystose) composition of foods and feeds.

摘要

An ion chromatographic method was developed to rapidly and accurately measure fructooligosaccharides (FOS) in selected food and feed ingredients ingested by man and animals. The 1-kestose (1-kestotriose; GF2), nystose (1,1-kestotetraose; GF3) and 1F-beta-fructofuranosylnystose (1,1,1-kestopentaose; GF4) content of a wide variety of foods (fruits and vegetables) and feedstuffs (including grains and forages) was determined. After extraction with water and appropriate filtration, samples were chromatographed, using an alkaline sodium acetate gradient, through an ion exchange column and guard fitted to a Dionex chromatography unit equipped with a pulsed electrochemical detector. All samples were prepared with and without spikes of standards to verifyrecovery and peak identification. Samples of the Compositae family were highest in total FOS followed by Allium species of the Amaryllidadeae family. The method provided excellent separation, recovery and quantification of the GFn units of FOS. It is concluded that accurate quantitation of FOS will allow more precise nutritional formulations to be developed with respect to inclusion of this functional food component in human and animal diets.