Influence of Two Fertilization Regimens on the Amounts of Organic Acids and Phenolic Compounds of Tronchuda Cabbage(Brassica oleracea L Var.costata DC)

摘要

A phytochemical study was undertaken on tronchuda cabbage(Brassica oleracea L.var.costata DC)cultivated under conventional and organic practices and collected at different times.Six organic acids(aconitic,citric,ascorbic,malic,shikimic,and fumaric acids)were identified and quantified by HPLC-UV.Qualitative and quantitative differences were noted between internal and external leaves.Analysis of the phenolics of the internal leaves was achieved by HPLC-DAD,and the phenolic profile obtained was revealed to be distinct from that of the external leaves.By this means were identified and quantified 11 compounds:3-p-coumaroylquinic acid,kaempferol 3-OMICRON-sophoroside-7-OMICRON-glucoside,kaempferol 3-OMICRON-(caffeoyl)sophoroside-7-OMICRON-glucoside,kaempferol 3-OMICRON-(sinapoyl)sophoroside-7-OMICRON-glucoside,kaempferol 3-OMICRON-(feruloyl)sophoroside-7-OMICRON-glucoside,kaempferol 3-OMICRON-sophoroside,two isomeric forms of 1,2-disinapoylgentiobiose,1-sinapoyl-2-feruloylgentiobiose,1,2,2'-trisinapoylgen-tiobiose,and 1,2'-disinapoyl-2-feruloylgentiobiose.In general,internal leaves exhibited more constant chemical profiles.