DISTRIBUTION OF SE IN SOYBEAN SAMPLES WITH DIFFERENT SE CONCENTRATION

摘要

Soybean samples from low-Se, Se-adequate and excessive Se regions were collected for determination of the distribution of Se in plant components. Chemical extraction indicated that most of Se was found in proteins, chitin, and polysaccharides. Based on chemical extraction and SDS-PAGE separation, the affinities of whey protein and, to a less extent, the affinity of the 11S globulin polypeptides were stronger for Se than that of 7S globulin polypeptides. The binding of Se to 7S and 11S under excessive Se environment was mostly nonspecific and could easily be detached from proteins during SDS-PAGE separation.