Synthesis of C-13-labeled patulin [4-hydroxy-4H-furo[3,2-c]pyran-2(6H)-one] to be used as internal standard in a stable isotope dilution assay

摘要

A synthetic route was established for the preparation of [C-13(2)]-4-hydroxy-4H-furo[3,2-c]pyran-2(6H)- one (patulin) to be used in a stable isotope dilution assay. Mass spectral analyses were performed using electron impact ionization (EI), negative electrospray ionization (ESI), collision-induced dissociation (CID), and atmospheric pressure ionization. Fragmentation routes in the EI mode and in CID were concluded and compared with each other. [References: 25]