Enantiomeric separation of Dns-DL-amino acids by gamma-cyclodextrin-modified micellar capillary electrophoresis

摘要

Cyclodextrin-modified micellar capillary electrophoresis (CD-MCE) was applied to enantiomeric separation of dansyl-derivatized (Dns)-DL-amino acids with sodium dodecyl sulfate (SDS). Effects of gamma-CD, capillary length, organic modifiers, and applied voltage on migration time, resolution (Rs), and separation efficiency were investigated. The results show that the migration behavior of Dns-DL-amino acid is related to the cavity sizes of CDs. gamma-CD has been found to be more effective in improving Rs and separation efficiency and shortening migration times, as compared to gamma-CD, as a chiral selector. Increase in the length of capillary and applied voltage are found to be beneficial for increasing Rs and separation efficiency. Addition of an organic modifier, such as 10% acetonitrile, to the background electrolyte solution helped to remarkably improve the enantioselectivity and separation of D-methionine and D-leucine. [References: 26]