Novel Method for the Authentication of Frigate Tunas(Auxis thazard and Auxis rochei)in Commereial Canned Products

摘要

A novel procedure for the authentication of frigate tunas(Auxis thazard and Auxis rochei)in commercially canned products has been developed.Three mitochondrial regions were simultaneously amplified by multiplex-Polymerase Chain Reaction,one corresponding to the small rRNA 12S subunit as a positive amplification control and two species-specific fragments corresponding to cytochrome b for A.rochei and ATPase 6 for A.thazard,respectively.Testing of two different detection systems revealed the fluorescence-based approach as the most sensitive.The results demonstrate that this rapid,low-cost methodology is a reliable molecular tool for direct application in the authentication of canned products.