Radioimmunoassay for cortisol in pig saliva and serum.

摘要

An "in-house" radioimmunoassay (RIA) procedure utilizing commercial sources of antiserum and radiolabel was developed for the simultaneous measurement of cortisol in serum and saliva from pigs. A commercial RIA kit (Incstar) for cortisol was used tovalidate the in-house procedure for serum measurements. The correlation coefficient between the Incstar kit and the in-house method for serum cortisol was r=0.94. The Incstar kit was modified to accommodate measurement of salivary cortisol concentrations and compared to the in-house procedure. The correlation between these procedures was r=0.81 and best described by a logarithmic (Ln) curve. Salivary cortisol measured by the in-house RIA were significantly higher than those obtained from the modified Incstar kit, a probable consequence of the different relative cross-reactions of the respective antisera to cortisone and a dilution effect on the kit standards.