NEW PROCEDURE FOR FUMONISINS B-1 AND B-2 IN CORN AND CORN PRODUCTS BY ION PAIR CHROMATOGRAPHY WITH O-PHTHALDIALDEHYDE POSTCOLUMN DERIVATIZATION AND FLUOROMETRIC DETECTION

摘要

Fumonisins are a group of naturally occurring mycotoxins produced by Fusarium moniliforme. An analytical method for fumonisins B-1 and B-2 in corn and related foods has been developed that utilizes ion pair chromatography with a postcolumn reaction technique. Samples were prepared and purified by tandem solid phase extraction columns (SAX and C-18). The fumonisins were derivatized with o-phthaldialdehyde and N-acetyl-L-cysteine in an in-line reaction coil. Ion pair liquid chromatography with fluorescence detection is used for the separation and detection. Absolute recoveries of fumonisins ranged from 54 to 110% at 0.04 and 0.08 mu g/g. The detection limit of this method is 20 ng. This procedure is relatively simple and provides a screening method for fumonisins in corn and related foods.