首页> 外文期刊>Journal of Agricultural and Food Chemistry >DELAYED RIPENING TOMATO PLANTS EXPRESSING THE ENZYME 1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID DEAMINASE .1. MOLECULAR CHARACTERIZATION, ENZYME EXPRESSION, AND FRUIT RIPENING TRAITS
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DELAYED RIPENING TOMATO PLANTS EXPRESSING THE ENZYME 1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID DEAMINASE .1. MOLECULAR CHARACTERIZATION, ENZYME EXPRESSION, AND FRUIT RIPENING TRAITS

机译:表达酶1-​​酰胺环丙烷1-羧酸脱氨酶的滞后番茄植株1。分子鉴定,酶表达和果熟性状

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摘要

Tomato plants that are delayed in fruit ripening have been developed by Agrobacterium tumefaciens-mediated transfer of a gene encoding 1-aminocyclopropane-1-carboxylic acid deaminase (ACCd) into the tomato genome. Two delayed ripening (DR) tomato lines are characterized in this paper. Line 8338, transformed by a double border plasmid vector, contains a single copy of the accd gene, and DNA outside the plasmid border sequences was not transferred to the plant genome. Line 5673, transformed by a single border plasmid vector, contains a single, complete copy of the accd gene plus tandem, incomplete copies of the gene. The mean expression levels of ACCd in fruit collected from four field trials were 39.4 and 20.6 mu g/g of fresh weight for lines 8338 and 5673, respectively. Fruit ethylene synthesis was significantly reduced, and time for fruit to ripen was extended for both DR tomato lines relative to the parental control line. Introduction of the accd gene and the delayed ripening trait into appropriate tomato varieties will potentially allow production of tomato fruit with superior taste quality.
机译:通过根癌农杆菌介导的将编码1-氨基环丙烷-1-羧酸脱氨酶(ACCd)的基因转移到番茄基因组中,已经开发出果实成熟延迟的番茄植株。本文介绍了两种延迟成熟(DR)番茄品系。由双边界质粒载体转化的品系8338,包含一个单拷贝的accd基因,质粒边界序列外的DNA没有转移到植物基因组中。由单一边界质粒载体转化的品系5673包含accd基因的单个完整副本以及该基因的串联非完整副本。从四个田间试验收集到的果实中,ACCd的平均表达水平分别为8338和5673系的39.4和20.6μg / g鲜重。相对于亲本对照品系,两个DR番茄品系的果实乙烯合成均显着减少,并且果实成熟的时间延长。将accd基因和延迟的成熟性状引入适当的番茄品种中,将有可能使番茄果实的口味质量更高。

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