Determination of hydrazine by liquid chromatography with preliminary derivatization with naphthalene-2,3-Dialdehyde

摘要

A method is proposed for the quantification of hydrazine by reversed-phase chromatography after its derivatization with naphthalene-2,3-dialdehyde. The conditions of derivatization and the chromatography separation on a Zorbax Eclipse XDB-C8 column in the gradient mode are optimized. The derivatization and chromatography analysis take 1 and 16 min, respectively. If fluorimetry detection (λ _(ex) = 273 nm, λ _(em) = 500 nm) is used and the injection volume is 100 μL, the detection limit is 0.05 μg/L. The procedure is applicable to the quantification of hydrazine in natural waters and soil extracts. A simple and rapid procedure is elaborated for the determination of 0.1-50 μg/L hydrazine in natural waters, RSD = 12% (n = 3).