Determination of phenolic acids and a flavonoid in Eleusine coracana (L.) by semi-preparative HPLC photo diode array detector

摘要

Semi preparative HPLC procedure is proposed for the determination of six phenolic acids and one flavonoid in Eleusine coracana (L.) with gradient elution and photodiode array detection. The influence of composition of the mobile phase concentration of the mix modifier and temperature on the separation of gallic acid, p-hydroxybenzoic, vanillic, caffeic, ferulic, and cinnamic acids and quercetin for 90 min is studied. The procedure was applied for the separation of phenolic compounds in methanolic extracts of Eleusine coracana (L.). The lower limits of quantification of phenolic compounds and the flavonoid are 0.050-0.150 mu g/mL.