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首页> 外文期刊>Journal of Analytical Toxicology >Rapid quantification of buprenorphine-glucuronide and norbuprenorphine-glucuronide in human urine by LC-MS-MS.
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Rapid quantification of buprenorphine-glucuronide and norbuprenorphine-glucuronide in human urine by LC-MS-MS.

机译:通过LC-MS-MS快速定量人类尿液中的丁丙诺啡-葡糖醛酸和去甲丁啡-葡糖醛酸。

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摘要

A liquid chromatography-tandem mass spectrometry (LC-MS-MS) method was developed and validated for the determination of buprenorphine-glucuronide (BUP-G) and norbuprenorphine-glucuronide (NBUP-G) in human urine. The method included a dilution step followed by filtration through a Mini-Uniprep Filter and direct injection onto the LC column. The analytes were quantified in multiple reactions monitoring mode using one transition ion. Norbuprenorpine-d(3) (NBUP-d(3)) was used as the internal standard. The concentration ranges were 6-161 ng/mL for BUP-G and 12-295 ng/mL for NBUP-G. Recoveries determined after filtration for the analytes were 75%. The between-day precision of the method was in the range of 4.8-11%. The limits of quantification were found to be 4.6 ng/mL for BUP-G and 11.8 ng/mL for NBUP-G. Approximately 1000 samples from law enforcement, prison inmates, probation services, and hospitals were analyzed by the presented method. The ratios of drug glucuronides versus creatinine were calculated for a selection of samples (n = 151), where there was information on treatment with buprenorphine between 16 and 20 mg/day. The majority (86%) of the samples had a ratio of BUP-G/creatinine below 570 microg/g, and 76% of the samples had NBUP-G/creatinine lower than 1060 microg/g. The LC-MS-MS method proved to be robust and specific for the determination of BUP-G and NBUP-G in urine.
机译:建立了液相色谱-串联质谱(LC-MS-MS)方法并验证了其在人尿中丁丙诺啡-葡糖醛酸苷(BUP-G)和去甲丁丙诺啡-葡糖醛酸苷(NBUP-G)的测定。该方法包括稀释步骤,然后通过Mini-Uniprep过滤器过滤并直接进样到LC色谱柱上。使用一个过渡离子以多种反应监测模式对分析物进行定量。 Norbuprenorpine-d(3)(NBUP-d(3))被用作内标。 BUP-G的浓度范围为6-161 ng / mL,NBUP-G的浓度范围为12-295 ng / mL。过滤后测定的分析物回收率为75%。该方法的日间精度在4.8-11%的范围内。发现BUP-G的定量限为4.6 ng / mL,NBUP-G的定量限为11.8 ng / mL。通过本方法对来自执法部门,监狱囚犯,缓刑部门和医院的大约1000个样本进行了分析。计算了所选样品(n = 151)中药物葡糖醛酸苷与肌酐的比例,其中有关于丁丙诺啡治疗的信息,范围为16至20 mg / day。大部分样品(86%)的BUP-G /肌酐比值低于570微克/克,而76%样品的NBUP-G /肌酐比值低于1060微克/克。 LC-MS-MS方法被证明是鲁棒且特异性强的尿液中BUP-G和NBUP-G的测定方法。

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