Anti-mutated citrullinated vimentin (anti-MCV) and anti-65kDa heat shock protein (anti-hsp65): New biomarkers in ankylosing spondylitis

摘要

Introduction: Citrullination as well as anti-citrullinated protein/peptide antibodies (ACPA) have been implicated in the pathogenesis of rheumatoid arthritis (RA). While ACPAs are specific and sensitive markers for RA, there have been hardly any reports regarding ACPAs in ankylosing spondylitis (AS). The possible role of antibodies to Mycobacterial 65. kDa heat shock protein (hsp65) has not been characterized in AS. As new laboratory biomarkers of AS are needed, we investigated the prevalence of anti-mutated citrullinated vimentin (MCV) and anti-hsp65 antibodies in AS. Methods: Altogether 43AS and 44healthy controls were included in the study. Anti-MCV and anti-hsp65 were determined in sera by commercial and in-house ELISA, respectively. Serum autoantibody levels were correlated with ESR, CRP, HLA-B27 status, smoking habits, pain intensity, BASDAI, BASFI and BASMI indices. Results: Patients with AS had significantly higher serum anti-MCV levels (17.3. U/mL, range: 8.3-31.5. U/mL) in comparison to healthy subjects (8.9. U/mL, range: 5.4-13.3. U/mL) (p< 0.01). Sixteen of the 43AS patients (37%) and none of the 44healthy controls (0%) were anti-MCV positive using the cut-off value recommended by the manufacturer (> 20. U/mL). The mean anti-hsp65 concentration in AS sera was 124.8. AU/mL (range: 27.2-1000. AU/mL), while controls exerted significantly lower anti-hsp65 levels (mean: 51.8 AU/mL; range: 22.5-88.5. AU/mL) (p< 0.001). Correlation analysis revealed that both anti-MCV positivity (r=0.613; p=0.012) and absolute serum anti-MCV levels (r=0.553; p=0.021) correlated with anti-hsp65 levels. Anti-MCV positivity also correlated with ESR (r=0.437; p=0.03). Conclusions: Anti-MCV and anti-hsp65 may be novel biomarkers in AS.