Determination of antifungal susceptibility of Aspergillus spp. responsible for otomycosis by E-test method Determination de la sensibilite a divers antifongiques d'Aspergillus sp.responsable d'otomycose par la methode du E-test

摘要

Objective. - In this study, we investigate in vitro activities of two azole derivates, ketoconazole and itraconazole, against Aspergillus spp. isolates causing otomycosis. Materials and methods. - Mycological analysis of samples from ear canals of patients was performed by culturing onto Sabouraud's dextrose agar and by evaluating microscopically. Aspergillus spp. was identified with colony morphology and microscopic appearance, and tested for susceptibility to ketoconazole and itraconazole by E-test method. A total of 52 isolates comprising 32 A. niger, 12 A. fumigatus and eight A. flavus were tested. Results. - Minimal Inhibitory Concentration (MIC) ranges of itraconazole and ketoconazole have been found as follows: 0.5-3 and 0.25-4 ng/ml for A. niger, 1-4 and 1.5-3.0 jig/ml for A. fumigatus and 0.75-1.5 and 0.25-0.75 mug/ml for A. flavus at 24 h. Conclusion. - It has been observed that both of the antifungal agents showed an in vitro activity against all Aspergillus spp. tested.