Evaluating the resistance of grapevines against anthracnose by pathogen inoculation, vineyard inspection, and bioassay with culture filtrate from Elsinoe ampelina.

摘要

Breeding of grape cultivars resistant to anthracnose is one of the most important breeding goals in Korea. In this study, evaluation of resistance using bioassay of grape leaves with culture filtrates from E. ampelina and their ethyl acetate extracts was compared with pathogen inoculation and field screening. To evaluate the resistance to anthracnose disease in grape germplasm, European grapes, American grapes, and Vitis hybrids were tested. Bioassay with culture filtrates produced by the pathogen showed that 'Black Eye', 'Mario', 'Niunai', 'Rizamat', and 'Rosario Bianco' were sensitive, while 'Campbell Early', 'Niagara', and 'Honey Red' were tolerant of anthracnose. In the evaluation by pathogen inoculation, some cultivars such as 'Black Swan', 'Rizamat', 'Rosario Bianco', and 'Kaiji' were susceptible, while others such as 'Campbell Early', 'Niagara', 'Sheridan', and 'Izumo Queen' were found to be resistant to anthracnose. Vineyard evaluation showed the same results. The results of bioassay with culture filtrates of the pathogen were consistent with those from pathogen inoculation and screening in the vineyard..