PEPTIDE MASS SPECTROMETRY BY IONIC PROBE ATTACHMENT IONIZATION USING NHS-TMPYBOX AND MAL-TMPYBOX

摘要

The application of a new ionization method that uses metal-complex-based ionization probes containing the 2,6-bis(oxazolinyl)-pyridine (pybox) ligand for bioactive peptides is presented. Bradykinin, angiotensin-I, angiotensin-II, and nociceptine as well as a couple of pentapeptides were analyzed. This method was proven to effectively ionize large complex molecules, including biomolecules.