HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC ENANTIO-SEPARATION BASED ON DIASTEREOMER FORMATION WITH NEW FLUORESCENT CHIRAL QUINOXALINES

摘要

Two kinds of chiral 2,3-dimorpholinoquinoxalines bearing L-proline and N-bromoacetylproline residues at C-6 position were newly synthesized.The derivatization of (+-)-naproxen,(+-)-ibuprofen,and (+-)-2-phenylpropionic acid with chiral quinoxalines afforded the diastereomers,which emitted an intense fluorescence.All the diastereomers were clearly separated within 16 min on reversed-phase (Finepak SIL C_(18)S) HPLC equipped with a fluorescence detector.The detection limit of the derivatized product was estimated to be 5 pmol/10 muL injection volume at S/N=5.Further,the quantum yield of the diastereomer was estimated to be 0.052 by using the relative quantum yield measurement with anthracene as a standard.