Evaluation of Polyhexamethylene Biguanide (PHMB) as a Disinfectant for Adenovirus

摘要

Importance: Swimming pools can be a vector for transmission of adenovirus ocular infections. Polyhexamethylene biguanide (PHMB) is a disinfectant used in swimming pools and hot tubs.Objective: To determine whether PHMB is an effective disinfectant against ocular adenovirus serotypes at a concentration used to disinfect swimming pools and hot tubs.Design: In vitro laboratory study.Interventions: The direct disinfecting activity of PHMB was determined in triplicate assays by incubating 9 human adenovirus types (1, 2, 3, 4, 5, 7a, 8, 19, and 37) with PHMB concentrations of 50 and 0 ppm (micro-grams per milliliter) for 24 hours at room temperature to simulate swimming pool temperatures or 40°C to simulate hot tub temperatures.